Publications

Below is the list of peer-reviewed articles, preprints, and book chapters authored or co-authored by members of the Doucet Lab, grouped by year.

2025

Nehmé, R.; Fortier, M.; Létourneau, M.; Fuselier, C.; Granger Joly De Boissel, P.; Dumoulin, A.; Guérin, B.; Dumulon-Perreault, V.; Ait-Mohand, S.; Sarrhini, O.; Larda, S. T.; Castellanos Villamizar, Y.; Bernier, M.; Porębska, N.; Opaliński, Ł.; Chatenet, D.; Doucet, N.; St-Pierre, Y. Development of Galectin-7-Specific Nanobodies: Implications for Immunotherapy and Molecular Imaging in Cancer. Journal of Medicinal Chemistry 2025, 68 (8), 8484–8496. https://doi.org/10.1021/acs.jmedchem.5c00071.
Granger Joly De Boissel, P.; Nehmé, R.; Fortier, M.; Létourneau, M.; Guérin, B.; Dumulon-Perreault, V.; Ait-Mohand, S.; Sarrhini, O.; Fuselier, C.; Dumoulin, A.; Chatenet, D.; Doucet, N.; St-Pierre, Y. Development and Use of a Galectin-1-Specific Nanobody for Tumor Imaging and Elucidating the Role of Galectin-1 in Cancer. ACS Pharmacology & Translational Science 2025, 8 (8), 2564–2574. https://doi.org/10.1021/acsptsci.5c00178.

2024

Tran, T. T. Q.; Narayanan, C.; Loes, A. N.; Click, T. H.; Pham, N. T. H.; Létourneau, M.; Harms, M. J.; Calmettes, C.; Agarwal, P. K.; Doucet, N. Ancestral Sequence Reconstruction Dissects Structural and Functional Differences among Eosinophil Ribonucleases. Journal of Biological Chemistry 2024, 300 (5), 107280. https://doi.org/10.1016/j.jbc.2024.107280.
De Oliveira Pereira, T.; Groleau, M.-C.; Doucet, N.; Déziel, E. Temperature-Responsive Control of Pseudomonas Aeruginosa Virulence Determinants through the Stabilization of Quorum Sensing Transcriptional Regulator RhlR. Microbiology May 13, 2024. Preprint. https://doi.org/10.1101/2024.05.13.593818.

2023

Lemay-St-Denis, C.; Alejaldre, L.; Jemouai, Z.; Lafontaine, K.; St-Aubin, M.; Hitache, K.; Valikhani, D.; Weerasinghe, N. W.; Létourneau, M.; Thibodeaux, C. J.; Doucet, N.; Baron, C.; Copp, J. N.; Pelletier, J. N. A Conserved SH3-like Fold in Diverse Putative Proteins Tetramerizes into an Oxidoreductase Providing an Antimicrobial Resistance Phenotype. Philosophical Transactions of the Royal Society B: Biological Sciences 2023, 378 (1871), 20220040. https://doi.org/10.1098/rstb.2022.0040.
Fuselier, C.; Dumoulin, A.; Paré, A.; Nehmé, R.; Ajarrag, S.; Granger Joly De Boissel, P.; Chatenet, D.; Doucet, N.; St-Pierre, Y. Placental Galectins in Cancer: Why We Should Pay More Attention. Cells 2023, 12 (3), 437. https://doi.org/10.3390/cells12030437.
Bernard, D. N.; Narayanan, C.; Hempel, T.; Bafna, K.; Bhojane, P. P.; Létourneau, M.; Howell, E. E.; Agarwal, P. K.; Doucet, N. Conformational Exchange Divergence along the Evolutionary Pathway of Eosinophil-Associated Ribonucleases. Structure 2023, 31 (3), 329–342.e4. https://doi.org/10.1016/j.str.2022.12.011.

2022

Lemay-St-Denis, C.; Doucet, N.; Pelletier, J. N. Integrating Dynamics into Enzyme Engineering. Protein Engineering, Design and Selection 2022, 35, gzac015. https://doi.org/10.1093/protein/gzac015.
Bui, D. T.; Li, Z; Kitov, P. I.; Han, L.; Kitova, E. N.; Fortier, M.; Fuselier, C.; Granger Joly De Boissel, P.; Chatenet, D.; Doucet, N.; Tompkins, S. M.; St-Pierre, Y.; Mahal, L. K.; Klassen, J. S. Quantifying Biomolecular Interactions Using Slow Mixing Mode (SLOMO) Nanoflow ESI-MS. ACS Central Science 2022, 8 (7), 963–974. https://doi.org/10.1021/acscentsci.2c00215.

2021

Pham, N. T. H.; Létourneau, M.; Fortier, M.; Bégin, G.; Al-Abdul-Wahid, M. S.; Pucci, F.; Folch, B.; Rooman, M.; Chatenet, D.; St-Pierre, Y.; Lagüe, P.; Calmettes, C.; Doucet, N. Perturbing Dimer Interactions and Allosteric Communication Modulates the Immunosuppressive Activity of Human Galectin-7. Journal of Biological Chemistry 2021, 297 (5), 101308. https://doi.org/10.1016/j.jbc.2021.101308.
Dionne, U.; Bourgault, É.; Dubé, A. K.; Bradley, D.; Chartier, F. J. M.; Dandage, R.; Dibyachintan, S.; Després, P. C.; Gish, G. D.; Pham, N. T. H.; Létourneau, M.; Lambert, J.-P.; Doucet, N.; Bisson, N.; Landry, C. R. Protein Context Shapes the Specificity of SH3 Domain-Mediated Interactions in Vivo. Nature Communications 2021, 12 (1), 1597. https://doi.org/10.1038/s41467-021-21873-2.

2020

Rueda, A. M.; López De Los Santos, Y.; Vincent, A. T.; Létourneau, M.; Hernández, I.; Sánchez, C. I.; Molina V., D.; Ospina, S. A.; Veyrier, F. J.; Doucet, N. Genome Sequencing and Functional Characterization of a Dictyopanus Pusillus Fungal Enzymatic Extract Offers a Promising Alternative for Lignocellulose Pretreatment of Oil Palm Residues. PLoS ONE 2020, 15 (7), e0227529. https://doi.org/10.1371/journal.pone.0227529.
Robitaille, S.; De Los Santos, Y. L.; Groleau, M.-C.; Jean-Pierre, F.; Doucet, N.; Perreault, J.; Déziel, E. An Experimentally Evolved Variant of RsmA Confirms Its Central Role in the Control of Pseudomonas Aeruginosa Social Motility. Microbiology July 15, 2020. Preprint. https://doi.org/10.1101/2020.07.15.203992.
Parent-Michaud, M.; Dufresne, P. J.; Fournier, E.; Folch, B.; Martineau, C.; Moreira, S.; Doucet, N.; De Repentigny, L.; Dufresne, S. F. Prevalence and Mechanisms of Azole Resistance in Clinical Isolates of Aspergillus Section Fumigati Species in a Canadian Tertiary Care Centre, 2000 to 2013. Journal of Antimicrobial Chemotherapy 2020, 75 (4), 849–858. https://doi.org/10.1093/jac/dkz534.
Narayanan, C.; Bernard, D. N.; Létourneau, M.; Gagnon, J.; Gagné, D.; Bafna, K.; Calmettes, C.; Couture, J.-F.; Agarwal, P. K.; Doucet, N. Insights into Structural and Dynamical Changes Experienced by Human RNase 6 upon Ligand Binding. Biochemistry 2020, 59 (6), 755–765. https://doi.org/10.1021/acs.biochem.9b00888.
López De Los Santos, Y.; Bernard, D. N.; Egesborg, P.; Létourneau, M.; Lafortune, C.; Cuneo, M. J.; Urvoas, A.; Chatenet, D.; Mahy, J.-P.; St-Pierre, Y.; Ricoux, R.; Doucet, N. Binding of a Soluble Meso-Tetraarylporphyrin to Human Galectin-7 Induces Oligomerization and Modulates Its Pro-Apoptotic Activity. Biochemistry 2020, 59 (48), 4591–4600. https://doi.org/10.1021/acs.biochem.0c00736.
Kariyawasam, K.; Ghattas, W.; De Los Santos, Y. L.; Doucet, N.; Gaillard, S.; Renaud, J.; Avenier, F.; Mahy, J.; Ricoux, R. Artificial Iron Hydrogenase Made by Covalent Grafting of Knölker’s Complex into Xylanase: Application in Asymmetric Hydrogenation of an Aryl Ketone in Water. Biotechnology and Applied Biochemistry 2020, 67 (4), 563–573. https://doi.org/10.1002/bab.1906.
Golbaghi, G.; Pitard, I.; Lucas, M.; Haghdoost, M. M.; De Los Santos, Y. L.; Doucet, N.; Patten, S. A.; Sanderson, J. T.; Castonguay, A. Synthesis and Biological Assessment of a Ruthenium(II) Cyclopentadienyl Complex in Breast Cancer Cells and on the Development of Zebrafish Embryos. European Journal of Medicinal Chemistry 2020, 188, 112030. https://doi.org/10.1016/j.ejmech.2019.112030.
Golbaghi, G.; Groleau, M.; López De Los Santos, Y.; Doucet, N.; Déziel, E.; Castonguay, A. Cationic RuII Cyclopentadienyl Complexes with Antifungal Activity against Several Candida Species. ChemBioChem 2020, 21 (21), 3112–3119. https://doi.org/10.1002/cbic.202000254.
Agarwal, P. K.; Bernard, D. N.; Bafna, K.; Doucet, N. Enzyme Dynamics: Looking Beyond a Single Structure. ChemCatChem 2020, 12 (19), 4704–4720. https://doi.org/10.1002/cctc.202000665.

2019

Santos, Y. L. D. L.; Chew-Fajardo, Y. L.; Brault, G.; Doucet, N. Dissecting the Evolvability Landscape of the CalB Active Site toward Aromatic Substrates. Scientific Reports 2019, 9 (1), 15588. https://doi.org/10.1038/s41598-019-51940-0.
Hudon Thibeault, A.-A.; López De Los Santos, Y.; Doucet, N.; Sanderson, J. T.; Vaillancourt, C. Serotonin and Serotonin Reuptake Inhibitors Alter Placental Aromatase. The Journal of Steroid Biochemistry and Molecular Biology 2019, 195, 105470. https://doi.org/10.1016/j.jsbmb.2019.105470.
Golbaghi, G.; Haghdoost, M. M.; Yancu, D.; López De Los Santos, Y.; Doucet, N.; Patten, S. A.; Sanderson, J. T.; Castonguay, A. Organoruthenium(II) Complexes Bearing an Aromatase Inhibitor: Synthesis, Characterization, in Vitro Biological Activity and in Vivo Toxicity in Zebrafish Embryos. Organometallics 2019, 38 (3), 702–711. https://doi.org/10.1021/acs.organomet.8b00897.
Gobeil, S. M. C.; Ebert, M. C. C. J. C.; Park, J.; Gagné, D.; Doucet, N.; Berghuis, A. M.; Pleiss, J.; Pelletier, J. N. The Structural Dynamics of Engineered β-Lactamases Vary Broadly on Three Timescales yet Sustain Native Function. Scientific Reports 2019, 9 (1), 6656. https://doi.org/10.1038/s41598-019-42866-8.
Dulcey, C. E.; López De Los Santos, Y.; Létourneau, M.; Déziel, E.; Doucet, N. Semi-rational Evolution of the 3-(3-hydroxyalkanoyloxy)Alkanoate (HAA) Synthase RhlA to Improve Rhamnolipid Production in Pseudomonas aeruginosa and Burkholderia glumae. The FEBS Journal 2019, 286 (20), 4036–4059. https://doi.org/10.1111/febs.14954.
Doucet, N. Enzyme Catalysis under Pressure. Nature Catalysis 2019, 2 (8), 646–647. https://doi.org/10.1038/s41929-019-0326-3.
Bafna, K.; Narayanan, C.; Chennubhotla, S. C.; Doucet, N.; Agarwal, P. K. Nucleotide Substrate Binding Characterization in Human Pancreatic-Type Ribonucleases. PLoS ONE 2019, 14 (8), e0220037. https://doi.org/10.1371/journal.pone.0220037.
Antunes, V. U.; Llontop, E. E.; Vasconcelos, F. N. D. C.; López De Los Santos, Y.; Oliveira, R. J.; Lincopan, N.; Farah, C. S.; Doucet, N.; Mittermaier, A.; Favaro, D. C. Importance of the Β5−β6 Loop for the Structure, Catalytic Efficiency, and Stability of Carbapenem-Hydrolyzing Class D β-Lactamase Subfamily OXA-143. Biochemistry 2019, 58 (34), 3604–3616. https://doi.org/10.1021/acs.biochem.9b00365.

2018

St-Pierre, Y.; Doucet, N.; Chatenet, D. A New Approach to Inhibit Prototypic Galectins. Trends in Glycoscience and Glycotechnology 2018, 30 (172), SE155–SE165. https://doi.org/10.4052/tigg.1730.1SE.
Narayanan, C.; Bernard, D. N.; Bafna, K.; Gagné, D.; Agarwal, P. K.; Doucet, N. Ligand-Induced Variations in Structural and Dynamical Properties Within an Enzyme Superfamily. Frontiers in Molecular Biosciences 2018, 5, 54. https://doi.org/10.3389/fmolb.2018.00054.
Narayanan, C.; Bernard, D. N.; Bafna, K.; Gagné, D.; Chennubhotla, C. S.; Doucet, N.; Agarwal, P. K. Conservation of Dynamics Associated with Biological Function in an Enzyme Superfamily. Structure 2018, 26 (3), 426–436.e3. https://doi.org/10.1016/j.str.2018.01.015.
Gagné, D.; Narayanan, C.; Bafna, K.; Charest, L.-A.; Agarwal, P. K.; Doucet, N. Correction to: Sequence-Specific Backbone Resonance Assignments and Microsecond Timescale Molecular Dynamics Simulation of Human Eosinophil-Derived Neurotoxin. Biomolecular NMR Assignments 2018, 12 (2), 365–367. https://doi.org/10.1007/s12104-018-9833-4.
Dionne, U.; Chartier, F. J. M.; López De Los Santos, Y.; Lavoie, N.; Bernard, D. N.; Banerjee, S. L.; Otis, F.; Jacquet, K.; Tremblay, M. G.; Jain, M.; Bourassa, S.; Gish, G. D.; Gagné, J.-P.; Poirier, G. G.; Laprise, P.; Voyer, N.; Landry, C. R.; Doucet, N.; Bisson, N. Direct Phosphorylation of SRC Homology 3 Domains by Tyrosine Kinase Receptors Disassembles Ligand-Induced Signaling Networks. Molecular Cell 2018, 70 (6), 995–1007.e11. https://doi.org/10.1016/j.molcel.2018.05.013.
Debbabi, S.; Groleau, M.-C.; Létourneau, M.; Narayanan, C.; Gosselin, L.-L.; Iddir, M.; Gagnon, J.; Doucet, N.; Déziel, E.; Chatenet, D. Antibacterial Properties of the Pituitary Adenylate Cyclase-Activating Polypeptide: A New Human Antimicrobial Peptide. PLoS ONE 2018, 13 (11), e0207366. https://doi.org/10.1371/journal.pone.0207366.

2017

Narayanan, C.; Gagné, D.; Reynolds, K. A.; Doucet, N. Conserved Amino Acid Networks Modulate Discrete Functional Properties in an Enzyme Superfamily. Scientific Reports 2017, 7 (1), 3207. https://doi.org/10.1038/s41598-017-03298-4.
Narayanan, C.; Bafna, K.; Roux, L. D.; Agarwal, P. K.; Doucet, N. Applications of NMR and Computational Methodologies to Study Protein Dynamics. Archives of Biochemistry and Biophysics 2017, 628, 71–80. https://doi.org/10.1016/j.abb.2017.05.002.
Gagné, D.; Narayanan, C.; Bafna, K.; Charest, L.-A.; Agarwal, P. K.; Doucet, N. Sequence-Specific Backbone Resonance Assignments and Microsecond Timescale Molecular Dynamics Simulation of Human Eosinophil-Derived Neurotoxin. Biomolecular NMR Assignments 2017, 11 (2), 143–149. https://doi.org/10.1007/s12104-017-9736-9.

2016

Nguyen-Thi, N.; Doucet, N. Combining Chitinase C and N-Acetylhexosaminidase from Streptomyces coelicolor A3(2) Provides an Efficient Way to Synthesize N-Acetylglucosamine from Crystalline Chitin. Journal of Biotechnology 2016, 220, 25–32. https://doi.org/10.1016/j.jbiotec.2015.12.038.
Narayanan, C.; Bernard, D.; Doucet, N. Role of Conformational Motions in Enzyme Function: Selected Methodologies and Case Studies. Catalysts 2016, 6 (6), 81. https://doi.org/10.3390/catal6060081.
Herrero, C.; Nguyen-Thi, N.; Hammerer, F.; Banse, F.; Gagné, D.; Doucet, N.; Mahy, J.-P.; Ricoux, R. Photoassisted Oxidation of Sulfides Catalyzed by Artificial Metalloenzymes Using Water as an Oxygen Source. Catalysts 2016, 6 (12), 202. https://doi.org/10.3390/catal6120202.
Gobeil, S. M. C.; Gagné, D.; Doucet, N.; Pelletier, J. N. 15N, 13C and 1H Backbone Resonance Assignments of an Artificially Engineered TEM-1/PSE-4 Class A β-Lactamase Chimera and Its Deconvoluted Mutant. Biomolecular NMR Assignments 2016, 10 (1), 93–99. https://doi.org/10.1007/s12104-015-9645-8.
Gagné, D.; Narayanan, C.; Nguyen-Thi, N.; Roux, L. D.; Bernard, D. N.; Brunzelle, J. S.; Couture, J.-F.; Agarwal, P. K.; Doucet, N. Ligand Binding Enhances Millisecond Conformational Exchange in Xylanase B2 from Streptomyces lividans. Biochemistry 2016, 55 (30), 4184–4196. https://doi.org/10.1021/acs.biochem.6b00130.
Agarwal, P. K.; Doucet, N.; Chennubhotla, C.; Ramanathan, A.; Narayanan, C. Conformational Sub-States and Populations in Enzyme Catalysis. In Methods in Enzymology; Elsevier, 2016; Vol. 578, pp 273–297. https://doi.org/10.1016/bs.mie.2016.05.023.

2015

Vladoiu, M. C.; Labrie, M.; Létourneau, M.; Egesborg, P.; Gagné, D.; Billard, É.; Grosset, A.-A.; Doucet, N.; Chatenet, D.; St-Pierre, Y. Design of a Peptidic Inhibitor That Targets the Dimer Interface of a Prototypic Galectin. Oncotarget 2015, 6 (38), 40970–40980. https://doi.org/10.18632/oncotarget.5403.
Tadevosyan, A.; Létourneau, M.; Folch, B.; Doucet, N.; Villeneuve, L. R.; Mamarbachi, A. M.; Pétrin, D.; Hébert, T. E.; Fournier, A.; Chatenet, D.; Allen, B. G.; Nattel, S. Photoreleasable Ligands to Study Intracrine Angiotensin II Signalling. The Journal of Physiology 2015, 593 (3), 521–539. https://doi.org/10.1113/jphysiol.2014.279109.
Ndoti-Nembe, A.; Vu, K. D.; Han, J.; Doucet, N.; Lacroix, M. Antimicrobial Effects of Nisin, Essential Oil, and γ-Irradiation Treatments against High Load of Salmonella typhimurium on Mini-carrots. Journal of Food Science 2015, 80 (7). https://doi.org/10.1111/1750-3841.12918.
Ndoti-Nembe, A.; Vu, K. D.; Doucet, N.; Lacroix, M. Antimicrobial Effects of Essential Oils, Nisin, and Irradiation Treatments against Listeria monocytogenes on Ready-to-Eat Carrots. Journal of Food Science 2015, 80 (4). https://doi.org/10.1111/1750-3841.12832.
Gagné, D.; Narayanan, C.; Doucet, N. Network of Long-range Concerted Chemical Shift Displacements upon Ligand Binding to Human Angiogenin. Protein Science 2015, 24 (4), 525–533. https://doi.org/10.1002/pro.2613.
Gagné, D.; French, R. L.; Narayanan, C.; Simonović, M.; Agarwal, P. K.; Doucet, N. Perturbation of the Conformational Dynamics of an Active-Site Loop Alters Enzyme Activity. Structure 2015, 23 (12), 2256–2266. https://doi.org/10.1016/j.str.2015.10.011.
Gagné, D.; Doucet, N. Sequence-Specific Backbone 1H, 13C, and 15N Resonance Assignments of Human Ribonuclease 4. Biomolecular NMR Assignments 2015, 9 (1), 181–185. https://doi.org/10.1007/s12104-014-9570-2.
Egesborg, P.; Carlettini, H.; Volpato, J. P.; Doucet, N. Combinatorial Active-site Variants Confer Sustained Clavulanate Resistance in BlaC β-lactamase from Mycobacterium tuberculosis. Protein Science 2015, 24 (4), 534–544. https://doi.org/10.1002/pro.2617.

2014

Thi, N. N.; Offen, W. A.; Shareck, F.; Davies, G. J.; Doucet, N. Structure and Activity of the Streptomyces coelicolor A3(2) β-N-Acetylhexosaminidase Provides Further Insight into GH20 Family Catalysis and Inhibition. Biochemistry 2014, 53 (11), 1789–1800. https://doi.org/10.1021/bi401697j.
Nguyen, T. T. N.; Folch, B.; Létourneau, M.; Truong, N. H.; Doucet, N.; Fournier, A.; Chatenet, D. Design of a Truncated Cardiotoxin-I Analogue with Potent Insulinotropic Activity. Journal of Medicinal Chemistry 2014, 57 (6), 2623–2633. https://doi.org/10.1021/jm401904q.
Grosset, A.-A.; Labrie, M.; Gagné, D.; Vladoiu, M.-C.; Gaboury, L.; Doucet, N.; St-Pierre, Y. Cytosolic Galectin-7 Impairs P53 Functions and Induces Chemoresistance in Breast Cancer Cells. BMC Cancer 2014, 14 (1), 801. https://doi.org/10.1186/1471-2407-14-801.
Gobeil, S. M. C.; Clouthier, C. M.; Park, J.; Gagné, D.; Berghuis, A. M.; Doucet, N.; Pelletier, J. N. Maintenance of Native-like Protein Dynamics May Not Be Required for Engineering Functional Proteins. Chemistry & Biology 2014, 21 (10), 1330–1340. https://doi.org/10.1016/j.chembiol.2014.07.016.
Brault, G.; Shareck, F.; Hurtubise, Y.; Lépine, F.; Doucet, N. Short-Chain Flavor Ester Synthesis in Organic Media by an E. Coli Whole-Cell Biocatalyst Expressing a Newly Characterized Heterologous Lipase. PLoS ONE 2014, 9 (3), e91872. https://doi.org/10.1371/journal.pone.0091872.

2013

Ndoti-Nembe, A.; Vu, K. D.; Doucet, N.; Lacroix, M. Effect of Combination of Essential Oils and Bacteriocins on the Efficacy of Gamma Radiation against Salmonella typhimurium and Listeria monocytogenes. International Journal of Radiation Biology 2013, 89 (10), 794–800. https://doi.org/10.3109/09553002.2013.797621.
Garénaux, A.; Houle, S.; Folch, B.; Dallaire, G.; Truesdell, M.; Lépine, F.; Doucet, N.; Dozois, C. M. Avian Lipocalin Expression in Chickens Following Escherichia coli Infection and Inhibition of Avian Pathogenic Escherichia coli Growth by Ex-FABP. Veterinary Immunology and Immunopathology 2013, 152 (1–2), 156–167. https://doi.org/10.1016/j.vetimm.2012.09.018.
Gagné, D.; Doucet, N. Structural and Functional Importance of Local and Global Conformational Fluctuations in the RNase A Superfamily. The FEBS Journal 2013, 280 (22), 5596–5607. https://doi.org/10.1111/febs.12371.
Folch, B.; Déziel, E.; Doucet, N. Systematic Mutational Analysis of the Putative Hydrolase PqsE: Toward a Deeper Molecular Understanding of Virulence Acquisition in Pseudomonas aeruginosa. PLoS ONE 2013, 8 (9), e73727. https://doi.org/10.1371/journal.pone.0073727.
Dulcey, C. E.; Dekimpe, V.; Fauvelle, D.-A.; Milot, S.; Groleau, M.-C.; Doucet, N.; Rahme, L. G.; Lépine, F.; Déziel, E. The End of an Old Hypothesis: The Pseudomonas Signaling Molecules 4-Hydroxy-2-Alkylquinolines Derive from Fatty Acids, Not 3-Ketofatty Acids. Chemistry & Biology 2013, 20 (12), 1481–1491. https://doi.org/10.1016/j.chembiol.2013.09.021.
Chatenet, D.; Folch, B.; Feytens, D.; Létourneau, M.; Tourwé, D.; Doucet, N.; Fournier, A. Development and Pharmacological Characterization of Conformationally Constrained Urotensin II-Related Peptide Agonists. Journal of Medicinal Chemistry 2013, 56 (23), 9612–9622. https://doi.org/10.1021/jm401153j.

2012

Nguyen, T. T. N.; Folch, B.; Létourneau, M.; Vaudry, D.; Truong, N. H.; Doucet, N.; Chatenet, D.; Fournier, A. Cardiotoxin-I: An Unexpectedly Potent Insulinotropic Agent. ChemBioChem 2012, 13 (12), 1805–1812. https://doi.org/10.1002/cbic.201200081.
Gagné, D.; Charest, L.-A.; Morin, S.; Kovrigin, E. L.; Doucet, N. Conservation of Flexible Residue Clusters among Structural and Functional Enzyme Homologues. Journal of Biological Chemistry 2012, 287 (53), 44289–44300. https://doi.org/10.1074/jbc.M112.394866.
Doan, N.-D.; Létourneau, M.; Vaudry, D.; Doucet, N.; Folch, B.; Vaudry, H.; Fournier, A.; Chatenet, D. Design and Characterization of Novel Cell-Penetrating Peptides from Pituitary Adenylate Cyclase-Activating Polypeptide. Journal of Controlled Release 2012, 163 (2), 256–265. https://doi.org/10.1016/j.jconrel.2012.08.021.
Clouthier, C. M.; Morin, S.; Gobeil, S. M. C.; Doucet, N.; Blanchet, J.; Nguyen, E.; Gagné, S. M.; Pelletier, J. N. Chimeric β-Lactamases: Global Conservation of Parental Function and Fast Time-Scale Dynamics with Increased Slow Motions. PLoS ONE 2012, 7 (12), e52283. https://doi.org/10.1371/journal.pone.0052283.
Brault, G.; Shareck, F.; Hurtubise, Y.; Lépine, F.; Doucet, N. Isolation and Characterization of EstC, a New Cold-Active Esterase from Streptomyces coelicolor A3(2). PLoS ONE 2012, 7 (3), e32041. https://doi.org/10.1371/journal.pone.0032041.
Allard, M.; Dupont, C.; Muñoz Robles, V.; Doucet, N.; Lledós, A.; Maréchal, J.; Urvoas, A.; Mahy, J.; Ricoux, R. Incorporation of Manganese Complexes into Xylanase: New Artificial Metalloenzymes for Enantioselective Epoxidation. ChemBioChem 2012, 13 (2), 240–251. https://doi.org/10.1002/cbic.201100659.

2011

Doucet, N.; Khirich, G.; Kovrigin, E. L.; Loria, J. P. Alteration of Hydrogen Bonding in the Vicinity of Histidine 48 Disrupts Millisecond Motions in RNase A. Biochemistry 2011, 50 (10), 1723–1730. https://doi.org/10.1021/bi1018539.
Doucet, N.; Loria, J. P. Relaxation Dispersion Studies of Enzyme Motions. In Advances in Biomedical Spectroscopy; IOS Press, 2011. https://doi.org/10.3233/978-1-60750-695-9-185.
Doucet, N. Can Enzyme Engineering Benefit from the Modulation of Protein Motions? Lessons Learned from NMR Relaxation Dispersion Experiments. Protein and Peptide Letters 2011, 18 (4), 336–343. https://doi.org/10.2174/092986611794653950.

2010

Doucet, N.; Jayasundera, T. B.; Simonović, M.; Loria, J. P. The Crystal Structure of Ribonuclease A in Complex with Thymidine-3′-monophosphate Provides Further Insight into Ligand Binding. Proteins: Structure, Function, and Bioinformatics 2010, 78 (11), 2459–2468. https://doi.org/10.1002/prot.22754.

2009

Doucet, N.; Watt, E. D.; Loria, J. P. The Flexibility of a Distant Loop Modulates Active Site Motion and Product Release in Ribonuclease A. Biochemistry 2009, 48 (30), 7160–7168. https://doi.org/10.1021/bi900830g.
De Wals, P.; Doucet, N.; Pelletier, J. N. High Tolerance to Simultaneous Active-site Mutations in TEM-1 β-lactamase: Distinct Mutational Paths Provide More Generalized β-lactam Recognition. Protein Science 2009, 18 (1), 147–160. https://doi.org/10.1002/pro.25.

2008

Doucet, N.; Pelletier, J. N. Gaining Insight into Enzyme Function through Correlation with Protein Motions. In Protein Engineering Handbook; Lutz, S., Bornscheuer, U. T., Eds.; Wiley, 2008; pp 187–211. https://doi.org/10.1002/9783527634026.ch7.

2007

Doucet, N.; Savard, P.-Y.; Pelletier, J. N.; Gagné, S. M. NMR Investigation of Tyr105 Mutants in TEM-1 β-Lactamase. Journal of Biological Chemistry 2007, 282 (29), 21448–21459. https://doi.org/10.1074/jbc.M609777200.
Doucet, N.; Pelletier, J. N. Simulated Annealing Exploration of an Active-site Tyrosine in TEM-1 β-lactamase Suggests the Existence of Alternate Conformations. Proteins: Structure, Function, and Bioinformatics 2007, 69 (2), 340–348. https://doi.org/10.1002/prot.21485.

2006

Doucet, N. Mutagenèse semi-aléatoire et analyse dynamique de la β-lactamase TEM-1 de Escherichia coli, Université de Montréal, 2006. Thesis. https://doi.org/10.71781/25789.

2005

Chica, R. A.; Doucet, N.; Pelletier, J. N. Semi-Rational Approaches to Engineering Enzyme Activity: Combining the Benefits of Directed Evolution and Rational Design. Current Opinion in Biotechnology 2005, 16 (4), 378–384. https://doi.org/10.1016/j.copbio.2005.06.004.

2004

Doucet, N.; De Wals, P.-Y.; Pelletier, J. N. Site-Saturation Mutagenesis of Tyr-105 Reveals Its Importance in Substrate Stabilization and Discrimination in TEM-1 β-Lactamase. Journal of Biological Chemistry 2004, 279 (44), 46295–46303. https://doi.org/10.1074/jbc.M407606200.