The Doucet Lab Dynamics-Guided Protein Engineering and Molecular Design

Research

Our lab investigates how proteins work, evolve, and can be redesigned for new molecular functions.

We combine structural biology, AI-guided protein engineering, biophysical characterization, and food & biomolecular chemistry to uncover mechanisms of action and create innovative biotechnological tools.

IMCM / MIMC – Molecular Interactions and Materials Characterization platform

We also manage and perform closely integrated research with the IMCM / MIMC (Molecular Interactions and Materials Characterization) platform at INRS.

This platform provides academic and industrial access to advanced structural biology, biophysics, and analytical tools for probing:

We work with the platform to design and execute rigorous experimental pipelines, from biophysical screening to high-resolution structural analysis, in support of both fundamental and applied research in small-molecule, materials, protein, and food/biomolecular chemistry.

Learn more about the IMCM / MIMC platform

Research programs at a glance

Structural biology & protein engineering

We study how conformational dynamics, molecular flexibility, and active-site architecture shape enzyme function.
Our work integrates:

We apply these approaches to biologically important enzymes, including ribonucleases and carbohydrate-binding proteins such as galectins.

SPASE – AI-guided protein design

SPASE (Soluble Protein Analog Selection Engine) is our in-house AI-assisted protein design platform. It integrates:

We use SPASE to design improved protein variants, stabilize difficult scaffolds, explore evolutionary trajectories, and engineer novel molecular functions.

Nanobody engineering & molecular therapeutics

We develop and characterize nanobodies targeting:

Our pipeline includes phage-display nanobody discovery with partners, NMR epitope mapping, biophysical affinity measurements, and structure-guided redesign.
These nanobodies act as inhibitors, conformational traps, and crystallization or NMR chaperones for challenging targets.

Biomolecular & food systems characterization

We apply high-resolution spectroscopy and chemometrics to complex biological matrices.
In collaboration with academic, industrial and municipal partners, we focus on:

This interdisciplinary program bridges environmental microbiology, analytical chemistry, and biomolecular modeling to understand how ecosystems shape food chemistry.

Tools & infrastructure

Our work is supported by state-of-the-art NMR facilities, crystallography and biophysics platforms, analytical chemistry infrastructure, and high-performance computing resources for AI-driven protein engineering.

We also maintain the SPASE server, which hosts internal pipelines for automated design and analysis.